Polymorphism in Genes of the Reactive Oxygen Species family (NADPH oxidase, SOD and GPx) and cerebrovascular stroke.

Abstract

Background: Stroke is a one of the neurological disease which is caused by alternation in genetic and environmental factors .Increased reactive oxygen species and oxidative stress are playing the major role in the development of stroke. Several studies have demonstrated the association of polymorphism of single gene responsible for oxidative stress but not any study conducted yet to evaluate the association of polymorphisms of genes of ROS pathway which ultimately cause development of oxidative stress. Thus, this study was conducted to investigate the association between the polymorphism of genes of ROS pathway (NADPH oxidase, SOD and GPx) and stroke. Method: A total of 674 patients and 308 controls subjects were recruited in this study. Genotyping of SOD1 (rs1041740), SOD2 (rs4880), NADPH oxidase (rs4673) and GPx1 (rs1050450) were performed by LightCycler real-time PCR (Roche, Germany) using LightSNiP reagents (coupled primer and probe ,TIBMOLBIO,Germany) and FastStart DNAMaster HybProbe ( Roche Diagnostics) while genotyping of NADPH oxidase (rs1049255) 2 and GPx4 (713041) were done by PCR-RFLP. All biochemical parameter were measured in automated clinical chemistry analyzer, at department of laboratory medicine, CBNU hospital.